Using this powerful tool, it makes it possible to dissociate contribution from the mitochondrial genes vs that of the nuclear genes. A cybrid is then a hybrid cell which mixes the nuclear genes from one cell with the mitochondrial genes from another cell. ![]() ![]() The rho-zero cells do retain mitochondria and can grow in rich culture medium with certain supplements. Rho-zero cells are cells which have been depleted of their own mitochondrial DNA by prolonged incubation with ethidium bromide, a chemical which inhibits mitochondrial DNA replication. A special case of cybrid formation involves the use of rho-zero cells as the whole cell partner in the fusion. This enucleation can be effected by simultaneous application of centrifugal force and treatment of the cell with an agent that disrupts the cytoskeleton. Cytoplasmic hybrid A cytoplasmic hybrid is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast.
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